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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 26;507:231–241. doi: 10.1016/j.virol.2017.04.014

Fig. 7. Effect of decreased DDX6 abundance on the interaction of miR-122 with HCV RNA at site 1, site 2 and site 1+2 in the 5′ UTR.

Fig. 7

Huh7 cells were transfected with control or DDX6-specific siRNAs and exogenous miR-122 duplexed RNA. These cells were electroporated with in vitro transcribed H77ΔE1-p7 RNA with mutations inhibiting the interaction of endogenous miR-122 with site 1 (p3), site 2 (p3-4) or site 1+2 (p3+p3-4). H77 RNA was analyzed by northern blot 5 days post-electroporation. (A–C) Schematic and northern blots of mutant HCV genomes bound by endogenous (green) and/or exogenous miR-122 (blue) at site 1 (A; p3), site 2 (B; p3-4) and site 1+2 (C; p3+p3-4). (D) Quantitation of each northern blot (Fig. 7A–C) from three independent experiments. (E) Quantitation of relative HCV RNA abundance following transfection of control and DDX6-specific siRNAs and p3 complementary miR-122, and electroporation of mutant H77ΔE1-p7 RNA with p3 mutations at site 1, site 2 and site 1+2. Effects of DDX6 depletion on each mutant genome were normalized HCV RNA abundance of the mutant HCV genomes in cells transfected with control siRNAs. Error bars display the mean ± SD.