Figure 4.

Fibrillation is highly affected by the solution composition. C-36 fibrillation kinetics (8 μM) and ThT endpoint signal showed a large effect of the buffer ion identity and the ionic strength for reactions carried out in NBS (a) or PS (b). All buffers were prepared to pH 7.42 and had approximate ionic strengths of I_PB = 49 mM, I_PBS = 200 mM, I_ABC = 20 mM, I_TB = 16 mM, I_TBS = 166 mM, I_HEPES = 7 mM, and I_MOPS = 11 mM. Colored error bars indicate the triplicate SD for each point. TBS is Tris-buffered saline, TB is Tris buffer, PBS is phosphate-buffered saline, PB is phosphate buffer, and ABC is ammonium bicarbonate buffer. See Materials and Methods for exact buffer compositions. To see this figure in color, go online.