Figure 5.

An exogenous glass bead surface stimulates nucleation pathways of C-36. (a) Shown here are ThT traces of 8 μM C-36 in PBS with the addition of the indicated number of silica glass beads. A semilog relationship between the number of glass beads and t_1/2 is observed (right). (b) Given here are normalized aggregate mass curves using the highest ThT level for each reaction. Global fits to the SP model are displayed with best fit values: n_c = 2, n₂ = 5, k₊k_n = 5.7⋅10⁷. The secondary nucleation term, k₊k₂, was individually fit to each curve and displayed a linear relationship to the number of glass beads (right). F_crit at which the two nucleation pathways contributed equally to new aggregate formation in the presence of one glass bead was 0.0038 (for m₀ = 8 μM). To see this figure in color, go online.