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. 2017 Aug 8;12(8):e0181191. doi: 10.1371/journal.pone.0181191

Fig 1. 4'-HW abrogated LPS-induced production level of NO and PGE2 through inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages.

Fig 1

(A) The chemical structure of 4'-HW. (B) Cytotoxicity in RAW 264.7 macrophages treated with 4'-HW (0.5, 1.5 and 15 μM) for 24 h. (C, D, E, F) Cells were treated with 0.5, 5 and 15 μM 4'-HW for 1h prior to the addition of LPS (100 ng/mL) for an 24 h incubation. NO and PGE2 levels were determined with Griess regent and a commercial kit, respectively. The protein level of iNOS and COX-2 was determined by Western blot analysis using specific antibodies. The immune-reactive bands were quantified using the NIH Image J software. (G and H) Cells were pretreated with 4'-HW for 1 h prior to the addition of LPS (100 ng/mL), and then cells were further incubated for 4 h. The mRNA levels of iNOS and COX-2 were determined by quantitative real-time PCR. The data shown represent the mean ± SD of three independent experiments. #p<0.05 vs the control group; *p<0.05 vs the LPS-treated group.