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. 2017 Aug 8;12(8):e0181191. doi: 10.1371/journal.pone.0181191

Fig 2. 4'-HW suppressed LPS-induced expression of pro-inflammatory cytokines in RAW 264.7 macrophages.

Fig 2

(A) The cells were treated with 0.5, 5 and 15 μM 4'-HW for 1 h prior to LPS stimulation (100 ng/mL, 4 h). The transcripts of TNF-α, IL-6 and IL-1β were determined by quantitative real-time PCR. (B) Cells were pretreated with 4'-HW for 1 h prior to stimulation with LPS (100 ng/mL) for 12 h, the Supernatant levels of TNF-α, IL-6 and IL-1β, determined by ELISA. The data shown represent the mean ± SD of three independent experiments. #p<0.05 vs the control group; *p<0.05 vs the LPS-treated group.