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. 2017 Aug 8;12(8):e0181191. doi: 10.1371/journal.pone.0181191

Fig 7. Effect of 4'-HW on LPS-induced acute lung injury in mice.

Fig 7

Mice (n = 6 per group) were injected with 4'-HW or vehicle for 12 h and 1 h respectively before LPS injection (3 mg/kg, Intratracheal injection). (A) Lungs from each experimental group were processed for histological evaluation at 6 h after the LPS challenge: (a) control, (b) LPS, (c) LPS + DEX (5 mg/kg), (d) LPS + 4'-HW (20 mg/kg), (e) LPS + 4'-HW (10 mg/kg). (B) The BALF was collected 6 h after LPS challenge to measure the number of total cells. (C) The BALF was collected 6 h after LPS challenge to measure the concentration of total proteins. (D) Effect of 4'-HW on the MPO activity in lung homogenates. (E) the levels of TNF-α, IL-6 and IL-1β in total RNA from lung tissue as determined by quantitative real-time PCR. (F) The BALF levels of TNF-α, IL-6 and IL-1β, determined by ELISA. #p<0.05 vs the control group; *p<0.05 vs the LPS-treated group.