Skip to main content
. 2017 Aug 8;12(8):e0182904. doi: 10.1371/journal.pone.0182904

Fig 2. Summary of phospho-OPN and TRAcP isoform data.

Fig 2

The TRAcP 5B/5A ratio (A) is lowered in RF+ RA and this correlates with the level of OPN’s phosphorylation (B), since TRAcP 5B is the primary phosphatase of OPN, its phosphorylation is decreased in RA. An image of phospho-OPN staining on western blot (C) is shown to highlight the differences, the photo was cropped to allow better visualization of the bands. In conclusion, we demonstrate that OPN is significantly more phosphorylated in RA than in OA synovia, and there are no significant differences between seropositive and seronegative groups (Fig 1A). TRAcP 5B/5A ratio in synovial fluid is increased in OA, and there is a significant negative correlation between the measured ratios and phospho-OPN levels.