Fig 5. Comparison of distribution of DE obtained using different approaches.

MA plots, with x-axis showing log₂ average abundances against OFC with an offset of 20 (panel A) and FC (panels B and C). The example shown is the 02H ± rivals DE comparison. The red line indicates 0 log₂ FC/OFC and the blue lines ±0.5 log₂ FC/OFC. Red data points represent the genes ‘called’ differentially expressed by each of the methods. Panel A shows the results for subsampling normalization with DE calculated using the hierarchical approach, Panel B for DEseq2 and Panel C for edgeR. Panel D shows a Venn diagram identifying the number of differentially expressed genes identified by two or more methods versus uniquely by each.