Fig 2. Polyfunctionality of TCR-transduced T cells.

After retroviral transduction, CD8⁺ and CD4⁺ T cells were separated by magnetic activated cell sorting (MACS). Transduced CD8⁺ T cells were identified with MHC Streptamers and CD4⁺ T cells with an antibody against the murine constant domain of the transduced TCR. 1x10⁵ T2 cells loaded with decreasing amounts of peptide were co-cultured with 1x10⁵ CD8⁺ or CD4⁺ T cells expressing (A) C18-specific, (B) S20-specific, or (C) S172-specific TCRs. Cytokine⁺ cells were detected by intracellular cytokine staining and are given as % of TCR-transduced T cells. Data are presented as mean values from triplicate (CD8⁺) or single (CD4⁺) co-cultures.