Table 1. Top sixty mecillinam resistance alleles^a.

rank	geneb	fold enrichmentc	rank	geneb	fold enrichmentc	rank	geneb	fold enrichmentc
1	yrfF*	3903	21	tusA**	339	41	crr**	133
2	fusA	3080	22	ubiD	306	42	waaP**	129
3	ileS	2106	23	mtn	234	43	arcA**	121
4	rnt**	1317	24	galU**	225	44	rlmH	120
5	ubiE*	1079	25	cydB**	220	45	crp*	116
6	sspA**	1054	26	ispA*	219	46	yigP	104
7	tufA**	836	27	rsfS	207	47	rfaH**	103
8	gmhB	831	28	mreB*	194	48	pspE	98
9	mrdA*	814	29	waaF	190	49	rpoN	88
10	mreC*	750	30	tusD	186	50	sapB**	77
11	rnhA	744	31	mnmA	181	51	yheO	69
12	slt*	712	32	lpp**	177	52	lipB	63
13	mrdB*	629	33	tusC	175	53	yaaY	60
14	ybeD**	543	34	ptsI	167	54	gcvR**	59
15	cydA	518	35	iscS**	161	55	ackA	59
16	efp**	476	36	cydD**	154	56	nlpD**	59
17	prc**	464	37	ychF**	152	57	yadD	58
18	ubiX*	440	38	aceF	144	58	lpd	58
19	ratA	406	39	hns	142	59	waaG	57
20	tusB	361	40	cysE*	141	60	aroK*	55

^a Top sixty resistance alleles from 10 μg/ml mecillinam plates.

^b Genes highlighted by *and ** indicate previously identified or confirmed new mecillinam resistance loci, respectively.

^c Fold enrichment was calculated as total transposon insertion reads for a given gene from mecillinam-treated samples divided by the equivalent number of reads from untreated samples.