FIG. 2.

Multimodal microscopy imaging of microchanneled alginate hydrogel scaffolds. (a) TPEF imaging at 800 nm excitation allows visualization of the scaffold and seeded fibroblasts. (b) OCM imaging provides structural contrast of the hydrogel scaffold. (c) TPEF imaging at 920 nm excitation isolates the cells from the scaffold. (d) The overlay of all imaging modalities provides the location of cells in the context of the microchannels within the scaffold microenvironment. White arrows correspond to locations of seeded cells in the microchanneled scaffolds. Color key: Green, TPEF at 800 nm; Red, TPEF at 920 nm from DiI labeled fibroblasts; Cyan, OCM. Scale bar is 250 μm. OCM, optical coherence microscopy; TPEF, two-photon-excited fluorescence. Color images available online at www.liebertpub.com/tec