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. 2001 Jul 31;98(17):9599–9604. doi: 10.1073/pnas.171311298

Figure 4.

Figure 4

Effect of NS5A on the NF-κB controlled luciferase expression. (A) Dose-dependent activation of NF-κB by NS5A. Huh-7 cells were cotransfected with increasing amounts of pCNS5A along with the wild-type or mutant NF-κB luciferase plasmids. (B) Calcium chelators inhibit NS5A-mediated NF-κB-controlled luciferase activity. Cells were transfected as described in A and treated with EGTA-AM (500 μM), TMB-8 (100 μM), and RR (30 μM) for various times of inhibition before luciferase activity. (C) Antioxidants inhibit NS5A-mediated NF-κB-controlled luciferase activity. Transfected cells were treated with NAC (30 mM) and PDTC (100 μM) for 6 h before preparing lysates for luciferase activity. Cells were cotransfected with NS5A and Mn-SOD expression vector along with the reporter plasmids.