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. 2001 Jul 31;98(17):9599–9604. doi: 10.1073/pnas.171311298

Figure 6.

Figure 6

HCV NS5A and nonstructural proteins induce the production of intracellular ROS/superoxide anion radicals. (A) Untransfected (control) or NS5A-transfected Huh-7 cells were treated with 4 μM of dihydroethidium for 45 min. Cells were harvested, and superoxide levels were measured by using flow cytometer with excitation emission at 605 nm. ROS/superoxide levels were assessed in untransfected (blue) and cells transfected with pCNS5A (red). (B) ROS/superoxide levels were determined in untransfected (blue) and cells transfected with pCMV729-3010 (red).