Figure 1.

GFP immunofluorescence observed in the olfactory bulb after intranasal administration of AAV9-GFP. Three-month-old mucopolysaccharidosis type I (MPS I) mice (n = 3) were infused intranasally with an AAV9-GFP vector. At 8 months of age, animals were sacrificed, and the tissues were harvested and evaluated for GFP expression. Extensive GFP-ir (immunoreactivity) is observed in many cells in both rostral and caudal regions of the nasal epithelium (A–C). In some cases, GFP-ir extends into the cilia of nasal epithelial cells (B, higher magnification in C). No labeling was seen in the nasal epithelium from control animals that did not receive virus (D). Extensive GFP-ir is also seen in the glomeruli of the olfactory bulb after intranasal delivery of AAV9-GFP (green; E and F). Structures in blue are background fluorescence imaged using filter set for UV. Scale bars A, B, and D = 50 μm, C = 20 μm, E = 400 μm, F = 100 μm.