FIG. 3.

Tg(Bre:GFP); Tg(acvr1l_Q204D-mCherry) embryos exhibit ventralized phenotypes and increased Tg(Bre:GFP) reporter expression. Tg(Bre:GFP) (A–C), Tg(Bre:GFP); Tg(acvr1l-mCherry) (D–F), and Tg(Bre:GFP); Tg(acvr1l_Q204D-mCherry) (G–I) embryos at 24 hpf, after 1 h of heat-shock at 5 hpf. Embryos were analyzed through fluorescent microscopy for mCherry (B, E, H) and GFP (C, F, I) fluorescence intensity, and through bright field microscopy for gross morphological phenotypes (A, D, G). n = 10 embryos each, for at least three replicate experiments. Scale bar is 200 μm. e, eye; GFP, green fluorescent protein; hpf, hour postfertilization; s, somites; y, yolk. Color images available online at www.liebertpub.com/zeb