FIG. 4.

Acvr1l^Q204D is expressed in heat-shocked adult zebrafish. After 3 months of once daily heat-shock for 1 h at 38°C, adult Tg(Bre:GFP) (A–C) and Tg(Bre:GFP); Tg(acvr1l_Q204D-mCherry) (D–F) zebrafish were characterized through bright field microscopy (A, D) and through fluorescent microscopy for mCherry (B, E) and GFP (C, F) fluorescence intensity (n = 5 adult zebrafish). (G) Western blots of protein extracts from the same animals were probed using anti-mCherry (Acvr1l-mCherry at 84 kDa and free mCherry at 27 kDa), anti-GFP (27 kDa), and anti-β-actin (43 kDa) antibodies (n = 2 replicate Western blots). Color images available online at www.liebertpub.com/zeb