Figure 4.

Clonogenic Cholangiocytes Are Interspersed within Normal Bile Ducts

(A) Representative image of Pkhd1l1 (red) and Sox9 (green) RNA FISH staining. Scale bar, 10 μm.

(B) Venn diagram of the Pkhd1l1⁺ cells among all Sox9⁺ duct cells.

(C) Frequency of Sox9 RNA signals per duct cell.

(D) Frequency of Pkhd1l1 RNA signals per duct cell; 60% of cells has either no or one hybridization signal, delineating the negative population.

n = 88 independent experiments from cells in four different mice. Biliary cells were identified by typical morphology in phase-contrast microscopy.