Figure 5.
Validation of candidate genes from small RNA sequencing of nine samples, from five unique cardiosphere-derived cell (CDC) donors and a fibroblast line. Four conditions were variable across each sample, including oxygen concentration (2%, 5% and 20%), days of conditioning (5 and 15 days), three different passages (3–5) and a fibroblast control. (a) The Cq values for each sample were plotted to show the fluctuation of expression across donors and conditions.Data were analysed using (b) NormFinder, (c) GeNorm, (d) BestKeeper, and (e) Delta Ct. (f) The weighted geometric mean of each of these samples was taken to provide a consolidated list of the most stable genes.