FIG. 1.

Residual hydrophile and lipophile antigenicity of bovine pericardium following antigen removal in the presence or absence of magnesium in solubilizing solutions. Residual hydrophile antigenicity does not change in response to presence or absence of magnesium (A). Representative western blot demonstrating that all treatment groups result in equal percentage reduction of antigenicity compared to their respective controls (B). Absence of magnesium from both hydrophile and lipophile solubilizing solutions results in a statistically significant reduction in residual lipophile antigenicity (C). Representative western blot demonstrating that compared to AR^+/+, AR^−/− treatment group results in a greater percentage reduction of antigenicity compared to respective control (D). *Signifies p < 0.05, L = Ladder, T = Treated group, and C = Control group (n = 12 per group). n.s. signifies no significant differences between groups.