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. 2017 Jul 1;23(13-14):609–621. doi: 10.1089/ten.tea.2016.0405

FIG. 2.

FIG. 2.

Antigens removed from scaffolds during sequential and differential solubilization steps of antigen removal. Absence of magnesium from hydrophile solubilizing solution alone (AR+/−) and hydrophile and lipophile solubilizing solutions (AR−/−) results in statistically significant increase in removal of antigens during the nuclease digestion step (A). Representative western blot demonstrating increase in antigens removed in absence of magnesium (B). Absence of magnesium from both hydrophile and lipophile solubilizing solutions (AR−/−) results in statistically significant increase in antigens removed in the TBS washout step (C). Representative western blot demonstrating increase in antigens removed in absence of magnesium (D). *Signifies p < 0.05, L = Ladder, T = Treated group, and C = Control group (n = 12 per group). TBS, tris-buffered saline.