FIG. 4.

DNA content removed from scaffolds during nuclease digestion (NUC) and Tris-buffered saline (TBS) washout steps. DNA removal during nuclease digestion was unaltered by presence or absence of magnesium (A). A trend toward progressively increasing DNA removal during TBS washout was evident in the absence of magnesium compared to its presence (B), although this finding failed to reach statistical significance (n = 12 per group). * signifies p < 0.05. n.s. signifies no significant differences between groups.