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. 2017 Jul 1;23(13-14):609–621. doi: 10.1089/ten.tea.2016.0405

FIG. 5.

FIG. 5.

Effect of magnesium presence or absence on protein removal during nuclease digestion and TBS washout steps. Nuclear-associated protein removal is greater during TBS washout than during nuclease digestion (Ai). Total protein removal is greater during TBS washout than during nuclease digestion (Aii). Absence of magnesium increased both histone (B) and nuclear-associated protein (C) removal during TBS washout. Results plotted in Log [10] scale. ****signifies p < 0.0001, ***signifies p < 0.005, **signifies p < 0.015, and *signifies p < 0.05 (n = 6 per group).