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. 2017 Jul 1;23(13-14):609–621. doi: 10.1089/ten.tea.2016.0405

FIG. 6.

FIG. 6.

Gross histological morphology of native and antigen removed bovine pericardium in presence or absence of magnesium. Preservation of histological ECM morphology and marked reduction in residual nuclei are evident in H&E staining compared to native tissue. Verhoeff van Gieson (VVG) staining demonstrates preservation of collagen and elastin structure following antigen removal. Collagen structure was unchanged in PSR stained samples. PSR birefringence indicated no change in type I (thick fibers, yellow-orange) and type III (thin fibers, green) collagen (A). Quantification of birefringence demonstrates no change in collagen alignment between native and antigen removed BP (B). (n = 6 per antigen removed group, n = 5 per native group). Polarized light scale bar represents 100 μm, otherwise scale bar represents 50 μm. BP, bovine pericardium; ECM, extracellular matrix; H&E, hematoxylin and eosin; PSR, Picro-Sirius Red. n.s. signifies no significant differences between groups.