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AP Co-localizes with OCT-4 Expression
Cropped image of mESCs cultured for 4 days in pluripotent conditions and stained for AP assay and the pluripotent marker OCT-4. The yellow arrow shows a portion of a colony differentiated, where no OCT-4 or AP staining is visible; the red arrow shows a pluripotent colony positive to OCT-4 and AP staining. Scale bar, 100 μm. AP, alkaline phosphatase.
