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. 2017 Aug 8;6(1):1359479. doi: 10.1080/20013078.2017.1359479

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of patient-derived glioblastoma stem-like cells (GSC)-released extracellular vesicles in vitro. (a) Representative transmission electronic microscopy (TEM) of GSCs cultivated under normal conditions. TEM images were artificially colourized with blue for nucleus, brown for cytoplasm and green for vesicle-like structures. (b) Schematic protocol of EV isolation by serial ultracentrifugation steps from the GSC-conditioned medium (serum-free and mitogen-free DMEM/F12). (c) Following purification, EVs were resuspended in 0.2-µm-filtered saline and characterized by qNano for particle concentration and diameter. (d,e) Representative TEM pictures of GSC#1-EVs either unlabelled or immunogold-labelled for CD63. (f) A MACSQuant Analyser Flow cytometer was used to evaluate Annexin V and CD63 on purified GSC-EVs. (g) Size diversity of GSC#1-derived EVs was analysed using confocal microscopy and super-resolution SIM microscopy. Representative pictures of CD63 staining are shown.