Figure 5.

EVs isolated from GBM patient circulating blood contain abundant VEGF-A. (a) Probability of survival after initial diagnosis in primary GBM patients expressing low (blue line) or high (red line) VEGF-A levels (n = 498). (b) Patient circulating EVs were isolated by successive ultracentrifugations from the serum of healthy donors (Ctl) or primary diagnosed GBM patients (GBM). VEGF-A levels were quantified by ELISA in circulating EVs from healthy (n = 20) or GBM patients (n = 4). **, p < 0.01. (c) Confocal microscopy of purified EVs from GBM patients stained for VEGF-A (green) and CD63 (red).