Figure 2.

Astrocytes from Adult hSOD1^G93A Mutant Mice Strongly Promote Protein and Neurofilament Inclusions in hMNs Compared with Postnatal hSOD1^G93A Astrocytes

hMNs co-cultured with mutant postnatal astrocytes showed limited formation of pNF-H, P62, and UBIQUITIN inclusions (A, B, C, G, H, and I). In contrast, these inclusions were significantly increased in co-cultures with mutant adult astrocytes (D, E, F, G, H, and I). Quantifications are presented as mean ± SEM. Data collected from three independent experiments each with triplicates. ^∗p < 0.05; ^∗∗p < 0.01. Mann-Whitney test. Scale bars, 100 μM.