Fig 4. Family-level bacterial composition of Rockefeller and Singapore female midguts.

(A) Family level composition of LB-cultivable bacteria in Rock and Sing: Using the same samples shown in the single time point bacterial load analysis presented in Fig 1, we identified distinct bacterial colony types cultured from each individual female midgut and sequenced the 16S rRNA gene from each isolate to identify colony type to family level. Bacterial family counts from individual females were combined post hoc within each combination of strain and feeding status to obtain an overall percentage of bacterial family within each treatment group. LB-cultivable composition data for the other three strains shown in Fig 1 can be found in S2 Fig. (B) Family level composition using 16S rRNA high-throughput sequencing. We used Illumina MiSeq technology to sequence the V3-V4 hypervariable regions of the 16S rRNA gene from a pooled subset of samples from those used in the single time point bacterial load analysis presented in Fig 1 to determine their family-level 16S midgut bacterial community profile. Only OTUs representing >1% of the total reads are represented here. Cyanobacteria are presented at the phylum level, as reads could not be classified accurately below this level.