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. 2017 Jul 28;13(7):e1006937. doi: 10.1371/journal.pgen.1006937

Fig 7. NIP is required to sustain JNK signaling during late regeneration.

Fig 7

(A-H) Confocal images of fluorescence from the TRE-red reporter for JNK signaling in w1118 (A-D) and mole02670/+ (E-H) regenerating discs at R0 (A,B), R24 (B,F), R48 (C,G) and R72 (D,H). (I) Quantification of fluorescence intensity of the TRE-red reporter in max projections of the confocal images at R0, because at this time point the epithelium cannot be distinguished from the debris. w1118 n = 10 discs, mole02670/+ n = 14 discs. (J) Quantification of fluorescence intensity of the TRE-red reporter in single slices of the confocal images through the regenerating epithelium at R24, R48, and R72. R24 w1118 n = 11 discs, mole02670/+ n = 11 discs. R48 w1118 = 14 discs, mole02670/+ n = 15 discs. R72 w1118 n = 11 discs, mole02670/+ n = 11 discs. (K) Regeneration assays using adult wing size to assess extent of regenerative growth in the imaginal discs in w1118, mole02670/+, pucE69/+, and mole02670/+;pucE69/+ animals. Two independent experiments, thus error bars are SD. w1118 n = 26 wings, mole02670/+ n = 83 wings, pucE69/+ n = 99 wings, and mole02670/+;pucE69/+ n = 95 wings. p<0.0001 for all comparisons using a chi-squared test. Dashed blue line outlines the wing primordium. Scale bars are 100 μm. Error bars are SEM unless otherwise noted. *p<0.05, **p<0.001, ***p<0.0001.