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. 2017 Jul 27;9(2):615–628. doi: 10.1016/j.stemcr.2017.06.012

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Single and Multiplexed IVT gRNA-Mediated Transcriptional Modulation

(A) Transient expression of IVT gRNAs for transcriptional modulation of SNAP91 (1 gRNA), CEP162 (six pooled gRNAs), and FUT9 (ten pooled gRNAs). Normalized relative mRNA levels (compared with GFP [SNAP91] or ten gRNAs targeting a nearby non-promoter non-coding region [CEP162 and FUT9]) following transfection of dCas9^VP64 NPCs with IVT gRNAs targeted upstream of the TSS for SNAP91, CEP162, and FUT9.

(B) Multiplexed pools of ten, five (two independent pools), and one IVT gRNA(s) for transcriptional modulation of FUT9.

Data are presented as means ± SEM (bar graph) from at least three independent biological replicates. Each biological replicate is depicted by a circle. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.