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The duplex-quadruplex junction of RNA Mango resembles a GAAA tetraloop. (a) Cartoon representation of the junction with one flanking Watson-Crick base pair from the duplex (gray), and adjacent residues from the G-quadruplex (G8 and G26). b) Hydrogen bonding pattern within the junction. (c) Junction of RNA Mango superimposed on a canonical GAAA tetraloop (gray; PDB 4FNJ; ref. 20).
