Figure 4.

Sox2 Lineage Ablation Leads to Regression of Pre-existing BCa

(A) The G/B ratio was calculated as (gross bladder weight/body weight) × 100% and analyzed with Student’s t test for both groups; n = 4, ^∗p < 0.05.

(B) Quantitative measurement of SOX2, Ki67, KRT14, and CD44v6 expression in bladder tumor tissues (BCa) from Sox2^CreER;R26^tdTomato(Ctrl) or Sox2^CreER;R26^DTA(DTA) mice. Three different areas from each mouse (4 mice per group) were randomly picked, and the intensity of immunostaining was quantitatively measured using Image-Pro Plus 6.0 image analysis software. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p<0.001, Student’s t test.

(C) Representative histopathology and immunohistochemical staining of tumor tissues with indicated antibodies are shown.

(D) Tumor image showing secondary recipients 3 weeks after subcutaneous injection of the same number of tumor cells from Sox2 Tom or Sox2 DTA mice into immunodeficient mice.

Scale bars, 50 μm.