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Representative sodium concentration profiles at the hypotonic side of planar membranes. The solute polarization increased with an increase in the protein lipid ratio. (Inset) The corresponding increase in the water permeability is shown. At 23°C, it was equal to 20 μm/s in the absence of the protein (dashed line) and reached 54 μm/s after the reconstitution of AqpZ at a mass ratio of 1:100. The osmotic flux was driven by 1 M urea. pH was 7.5.
