Figure 1.

Human Oligodendrocyte Progenitor Cell Differentiation In Vitro

(A and B) Human PDGFαR ⁺ OPCs were cultured for 4 days in serum-free medium with PDGF-AA (A) or in the absence of mitogens (no growth factors [noGF]) (B). Cultures were stained for immature oligodendrocyte marker O4 (green). Scale bar, 25 μm.

(C) O4 quantification (mean ± SEM, n = 4 separate human samples). ^∗∗p < 0.001, t test.

(D) qPCR of MBP and CSPG4 (log₂ fold change relative to day 1 noGF, mean ± SEM, n = 4 human samples).

(E) Heatmap of OPC- and oligodendrocyte-enriched genes.