Figure 6.

GNB4/G_β4 Overexpression Induces MAPK Signaling

Fetal PDGFαR⁺ hOPCs were cultured and infected with intracellular Ca²⁺ reporter GCaMP6s. Following GNB4 or mCherry overexpression, time-lapse microscopy of Ca²⁺ response after Oxo-M treatment was recorded and analyzed.

(A) Individual responsive cell Ca²⁺ measurements for average spike amplitude (left), total number of peaks (center), and frequency (right), along with their box plot. GNB4 overexpression did not significantly change Ca²⁺ response (p > 0.05, n > 90 cells per condition, two-way ANOVA with Tukey’s HSD post test, n = 3).

(B) hOPC signaling was measured using luciferase-based reporter after infection with GNB4 or mCherry lentivirus. Activity is reported as fold change in luminescence compared with negative control. ^∗∗p < 0.01, ^∗∗∗p < 0.001 (n = 3), two-way ANOVA with Bonferroni post test.