Figure 2. Photostimulation of LC terminals in the BLA releases norepinephrine.
(A) LC neuron firing reliably to 10 Hz optical stimulation (CC=whole cell current clamp). (B) Fast scan cyclic voltammetry (FSCV) schematic. (C–D) Oxidative and reductive currents (scale bar 2 s by 0.4 nA), with representative cyclic voltammograms (inset) and representative color plots (below) in response to photostimulation are attenuated by reserpine (1 μM). Color plots for baseline and after reserpine (1 µM): Files were collected over 15 s (X-axis) where the carbon fiber microelectrode was ramped with a triangular waveform from −0.4V to 1.3V and back to −0.4V at 400 V/S (Y-axis) and sampled at 10 Hz. 10 Hz, 473 nm blue LED stimulation onset at 2 s. Oxidative currents (nA) are positive in direction and reductive currents are negative (see color coded scale bar on right). (E) Attenuation in NE oxidative current in response to reserpine (1 μM) n = 3 pairs; mean ± S.E.M). (F) Average of first 20 min and last 15 min in (E) (Data represented as mean ± SEM, Paired Student’s t-tests to baseline, Mean difference = 68.56, t(2) = 18.75, **p=0.0028, 95% CI [52.82 to 84.29]).