Figure 5. Co-divergence in TF occupancy across evolution can reveal potential cooperative interactions.
(a) Pair-wise co-association of TF/time point in D. melanogaster (left) and D. virilis (right). Co-association significances correspond to Z-Score denoting deviation from random expectation ranging from red (lower than random expectation denoting lack of co-association) to blue (higher than random expectation denoting strong co-association). (b) Above table, schematic representation of the 4 categories of binding events affecting orthologous CRM pairs. Below, table indicates the number of CRM pairs in each category with p-values (Fisher exact tests). Significant cases (p<=0.05) are highlighted in red. (c) Association test between the loss of TF1 occupancy (TF1 ChIP, rows) and the loss of TF2 motifs (TF2 STAP, column). Significant associations between pairs are indicated (Fisher exact tests). (d) Occupancy of orthologous enhancers with co-divergent binding. Binding of both Tin and Bap are lost in D. vir (right) compared to D. mel (left). Track descriptions are as in Figure 2c. Orthologous CRM region is highlighted in gray.