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. 2017 Aug 9;6:e26640. doi: 10.7554/eLife.26640

Author response image 1. Tryptophan-dansyl FRET assay.

Author response image 1.

(A) Emission scan resulting from excitation at 280nm of dansyl vesicles alone (blue trace) or vesicles in presence of SHIP2 Ptase (red trace). SHIP2 Ptase induces a reduction of the emission signal at the dansyl emission (maximum at 513nm). (B) Emission of dansyl vesicles alone (I0 set to 100%; blue bars) or in presence of SHIP2 Ptase or PtaseC2 (as% of I0; red bars). (C) The emission at 513nm of dansyl vesicles decreases linearly with increasing concentrations of SHIP2 Ptase.

DOI: http://dx.doi.org/10.7554/eLife.26640.036