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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Cell Biochem. 2017 May 15;118(10):3249–3259. doi: 10.1002/jcb.25973

Figure 5. LPC-induced activation of the CXCL10 gene promoter requires STAT1 binding.

Figure 5

(A) Schematic diagram of CXCL10 promoter constructs, including the three putative STAT1 binding sites. Mutations in the three putative STAT1 promoter binding sites sequences were generated using standard site-directed mutagenesis deletion techniques. Italic, uppercase letters in the sequences denote deletion sites. (B) Luciferase activity was determined using the LightSwitch Luciferase Assay System. After transfection with the respective luciferase constructs, Huh7 cells were treated with either vehicle or 40 μM LPC with or without 1 μM URMC-099 (URMC) or 100 μM STAT1 inhibitor fludarabine. Bar columns represent mean ± standard error of the mean. ** p < 0.01; * p < 0.05