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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Bone Miner Res. 2017 Jun 12;32(8):1761–1772. doi: 10.1002/jbmr.3167

Fig. 7.

Fig. 7

Illustration of theoretical acidification by osteocytes and visualization of the osteocyte environment. (A) Schematic image showing osteocyte with dendrites (blue) in bone (light grey) that is bathed in lacuno-canalicular fluid filled volume (yellow) and surrounded by perilacunar matrix (dark grey). (B) Representative BSEM images of acid etched murine bone specimens containing osteocyte lacuna. With short acid etching (left image) the perilacunar matrix is etched away but the removal of the neighboring and deeper bone matrix requires longer acid etching (right image).