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. Author manuscript; available in PMC: 2018 Apr 10.
Published in final edited form as: J Am Chem Soc. 2016 Oct 17;138(42):13774–13777. doi: 10.1021/jacs.6b05688

Figure 1.

Figure 1

Covalent capture of DUBs with Ub-Dha-N-methylamide (4). (A) C18 RP-HPLC chromatogram of 4. The asterisk indicates the injection peak. (B) ESI-MS of 4. Calculated [M + H]+ 8590.8 Da, observed 8593.1 ± 2.2 Da. (C) 15% SDS-PAGE of 4 incubated with USP2 (lane 4) and UCH-L3 (lane 5). (D) ESI-MS of UCH-L3 (top) and the UCH-L3–4 adduct (bottom). Calculated for UCH-L3 [M + H]+ 26 183 Da, observed 26 194.1 ± 4.4 Da. Calculated for UCH-L3–4 [M + H]+ 34 774 Da, observed 34 774.0 ± 4.9 Da.