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. Author manuscript; available in PMC: 2018 Apr 10.
Published in final edited form as: J Am Chem Soc. 2016 Oct 17;138(42):13774–13777. doi: 10.1021/jacs.6b05688

Figure 3.

Figure 3

Labeling of Usp15 with Ub-Dha-TRIM25 (9). (A) RP-HPLC chromatograph of 9. (B) ESI-MS of 9. Calculated 9966.5 Da, observed 9968.0 ± 1.4 Da. (C) Comparison of the CD spectra of 9 and Ub-Dha-N-methylamide. (D) 10% SDS-PAGE (left) and Western blot (right) of 9 incubated with full-length USP15. The USP15–9 adduct was detected with an anti-Ub antibody (P4D1, Santa Cruz Biotech).