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Increased total and HDM-specific IgG production in HDM-exposed offspring of HDM-exposed mothers. Offspring of PBS- and HDM-exposed mothers were treated with PBS or HDM as described in Methods. Serum isolated at time of sacrifice was assayed for (A) total IgE, (B) HDM-specific IgG1 and (C) HDM-specific IgG2a by ELISA. Lowest dilution on plate indicated. Mean + SEM shown. * and *** indicate p < 0.05 and p < 0.001. Data represent 7 – 18 mice pooled from 4 experiments.
