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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Feb 9;140(5):1404–1415.e9. doi: 10.1016/j.jaci.2016.12.972

Figure 6.

Figure 6

Maternal HDM exposure does not increased the magnitude of the Th2 response or magnitude of the Ig response following exposure of offspring to Aspergillus fumigatus. Offspring of PBS- and HDM-exposed mothers were exposed to PBS, HDM or AF extract at 6, 8 and 9 weeks of age. Single cell suspensions of lung cells were restimulated with ConA, and production of (A) IL-4, (B) IL-5, (C) IL-13 were assessed by ELISA. (D) The frequency of CD4+ cells producing IL-13 was assessed by flow cytometry. (E) Serum isolated at time of sacrifice was assayed for total IgE. Mean + SEM shown. Data represent 2 – 5 mice pooled from 2 experiments.