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Association kinetics of bombykol and BmorPBP. (A) Fluorescence decrease upon mixing bombykol and BmorPBPB at pH 7. The curve was fitted (reduced χ2, 0.76) by using two-exponential terms and used to calculate the association rate (kon). (B) At low pH, the association of bombykol and BmorPBPA is 10 times slower as indicated by the time course and the kinetic parameters obtained by curve fitting (reduced χ2, 0.15) with two-exponential terms.
