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. 2001 Aug 7;98(17):9636–9641. doi: 10.1073/pnas.141048498

Figure 4.

Figure 4

Activation of ERK2 and p38α MAP kinases by type I (open bars) and type II (filled bars) GnRH receptors in COS-7 cells. Stimulation of type I (a) and II (b) GnRH receptors with mammalian GnRH I, GnRH II, and antagonist 135-18. Inset panels depict anti-phospho-ERK2 immunoblotting of anti-myc COS-7 cell immunoprecipitates. (c Left) selective and time-dependent activation of p38α by GnRH II stimulation (100 nM) of type II GnRH receptor. Each bar represents the mean ± SEM for fold p38α stimulation measured in the cells for the specified time of ligand stimulation. Open bars represent p38α stimulation by treatment of COS-7 transfected with type I receptor and stimulated with GnRH I. Filled bars represent p38α stimulation by GnRH II treatment of COS-7 cells transfected with type II GnRH receptor. The Right panel demonstrates analogous data, but each bar (open, type I receptor stimulated by GnRH I; filled, type II receptor stimulated by GnRH II) represents mean ± SEM for phosphorylation of ERK2.