Figure 5.

TLR2-PI3K plays a non-redundant role in the murine and human macrophage response to P. gingivalis. WT murine BMM (A), RAW264.7 macrophages (B), and PMA-differentiated human THP-1 cells (C) were primed with IFN-γ. TLR2 and TLR4 were inhibited with blocking antibodies vs. isotype control (I.C.) for 1 h and PI3K was blocked with LY294 prior to challenge with P. gingivalis (MOI 10). Supernatants were collected after overnight incubation and TNF was measured by ELISA. Background (BG) represents IFN-γ primed cells not challenged with P. gingivalis. Cells challenged with P. gingivalis without any blocker are referred to in the graphs as (–). Representative graphs of >3 repeats are shown. ^*P ≤ 0.05, ^**P ≤ 0.01, ^***P ≤ 0.005.