Table 4.
List of differential expressed wheat miRNAs between different decelopment stage in sterile condition (SS) and fertile condition (FS) in this study.
| List of differential expressed wheat miRNAs between FS1 and SS1 | ||||||||
|---|---|---|---|---|---|---|---|---|
| miR_name | FS1-reads | SS1-reads | log2Ratio(SS1/FS1) normalized | p-value | q-value (Storey and Tibshirani, 2003) | UP_Down | Sequences | Length |
| tae-miR5062-5p | 18,519 | 21,541 | 0.551065615 | 0 | 0 | Up | UGAACCUUAGGGAACAGCCGCAU | 23 |
| tae-miR9677a | 49,526 | 30,779 | −0.353253159 | 1.40E-299 | 2.32E-298 | Down | UGGCCGUUGGUAGAGUAGGAGA | 22 |
| tae-miR9666b-3p | 3,630 | 4,316 | 0.582711903 | 1.14E-73 | 1.25E-72 | Up | CGGUUGGGCUGUAUGAUGGCGA | 22 |
| tae-miR5048-5p | 4,408 | 2,264 | −0.628263679 | 2.71E-68 | 2.25E-67 | Down | UUUGCAGGUUUUAGGUCUAAGU | 22 |
| tae-miR9666a-3p | 26,852 | 24,473 | 0.199148003 | 8.50E-61 | 5.63E-60 | Up | CGGUAGGGCUGUAUGAUGGCGA | 22 |
| tae-miR9662a-3p | 3,661 | 3,623 | 0.317933618 | 2.16E-21 | 1.02E-20 | Up | UUGAACAUCCCAGAGCCACCG | 21 |
| tae-miR9662b-3p | 3,661 | 3,623 | 0.317933618 | 2.16E-21 | 1.02E-20 | Up | UGAACAUCCCAGAGCCACCGG | 21 |
| tae-miR1130b-3p | 625 | 709 | 0.514916024 | 6.12E-11 | 2.53E-10 | Up | UCUUAUAUUAUGGGACGGAGG | 21 |
| tae-miR156 | 249 | 96 | −1.042052845 | 1.85E-10 | 6.80E-10 | Down | UGACAGAAGAGAGUGAGCACA | 21 |
| tae-miR9772 | 1,113 | 651 | −0.440737558 | 2.40E-10 | 7.96E-10 | Down | UGAGAUGAGAUUACCCCAUAC | 21 |
| tae-miR167a | 67,908 | 52,304 | −0.043673675 | 3.47E-09 | 1.04E-08 | Down | UGAAGCUGCCAGCAUGAUCUA | 21 |
| tae-miR171a | 678 | 372 | −0.532996066 | 4.13E-09 | 1.14E-08 | Down | UGAUUGAGCCGUGCCAAUAUC | 21 |
| tae-miR1120c-5p | 147 | 49 | −1.251975914 | 1.41E-08 | 3.58E-08 | Down | UAAUAUAAGAACGUUUUUGAC | 21 |
| tae-miR167c-5p | 68,035 | 52,499 | −0.04100059 | 2.76E-08 | 6.53E-08 | Down | UGAAGCUGCCAGCAUGAUCUGC | 22 |
| tae-miR164 | 774 | 779 | 0.342276348 | 2.64E-06 | 5.83E-06 | Up | UGGAGAAGCAGGGCACGUGCA | 21 |
| tae-miR396-5p | 66 | 19 | −1.46348002 | 1.84E-05 | 3.82E-05 | Down | AACUGUGAACUCGCGGGGAUG | 21 |
| tae-miR9677b | 74 | 26 | −1.176027061 | 0.000127882 | 0.000249069 | Down | CAGGGCGGGGAACAGGUGGCC | 21 |
| tae-miR9653a-3p | 34 | 7 | −1.947121333 | 0.000169241 | 0.00031131 | Down | UUUGAGACUUUGGCCAUGGCC | 21 |
| tae-miR159b | 179 | 204 | 0.521596151 | 0.000389703 | 0.000679112 | Up | UUUGGAUUGAAGGGAGCUCUG | 21 |
| tae-miR159a | 179 | 203 | 0.514506726 | 0.000475035 | 0.000786424 | Up | UUUGGAUUGAAGGGAGCUCUG | 21 |
| List of differential expressed wheat miRNAs between FS2 and SS2. | ||||||||
| miR_name | FS2-reads | SS2-reads | log2Ratio(SS2/FS2) normalized | p-value | q-value (Storey and Tibshirani, 2003) | UP_Down | Sequences | Length |
| tae-miR9677a | 37,359 | 20,970 | −0.717174926 | 0 | 0 | Down | UGGCCGUUGGUAGAGUAGGAGA | 22 |
| tae-miR9666a-3p | 35,543 | 22,469 | −0.545675488 | 0 | 0 | Down | CGGUAGGGCUGUAUGAUGGCGA | 22 |
| tae-miR5062-5p | 16,240 | 23,628 | 0.656899936 | 0 | 0 | Up | UGAACCUUAGGGAACAGCCGCAU | 23 |
| tae-miR9672b | 45,656 | 53,013 | 0.331495768 | 0 | 0 | Up | UACCACGACUGUCAUUAAGCA | 21 |
| tae-miR9662a-3p | 3,721 | 4,830 | 0.49228685 | 1.78E-56 | 2.46E-55 | Up | UUGAACAUCCCAGAGCCACCG | 21 |
| tae-miR9662b-3p | 3,721 | 4,830 | 0.49228685 | 1.78E-56 | 2.46E-55 | Up | UGAACAUCCCAGAGCCACCGG | 21 |
| tae-miR9663-5p | 961 | 1,547 | 0.802818912 | 3.60E-43 | 4.27E-42 | Up | AAGCGUAGUCGAACGAAUCUG | 21 |
| tae-miR9666b-3p | 4,755 | 5,616 | 0.356051646 | 9.14E-37 | 9.48E-36 | Up | CGGUUGGGCUGUAUGAUGGCGA | 22 |
| tae-miR9672a-3p | 1,209 | 1,710 | 0.616136132 | 1.57E-30 | 1.45E-29 | Up | CCACGACUGUCAUUAAGCAUC | 21 |
| tae-miR5048-5p | 3,052 | 3,677 | 0.384728266 | 4.59E-28 | 3.81E-27 | Up | UUUGCAGGUUUUAGGUCUAAGU | 22 |
| tae-miR9670-3p | 547 | 855 | 0.760337639 | 1.65E-22 | 1.25E-21 | Up | AGGUGGAAUACUUGAAGAAGA | 21 |
| tae-miR164 | 874 | 526 | −0.616616429 | 3.22E-15 | 2.23E-14 | Down | UGGAGAAGCAGGGCACGUGCA | 21 |
| tae-miR9674b-5p | 5,067 | 4,037 | −0.21189419 | 1.87E-12 | 1.15E-11 | Down | AUAGCAUCAUCCAUCCUACCC | 21 |
| tae-miR171a | 549 | 750 | 0.566038498 | 1.94E-12 | 1.15E-11 | Up | UGAUUGAGCCGUGCCAAUAUC | 21 |
| tae-miR396-5p | 69 | 16 | −1.992570405 | 1.78E-08 | 9.85E-08 | Down | AACUGUGAACUCGCGGGGAUG | 21 |
| tae-miR9772 | 854 | 1,020 | 0.372215229 | 2.34E-08 | 1.21E-07 | Up | UGAGAUGAGAUUACCCCAUAC | 21 |
| tae-miR1135 | 312 | 423 | 0.555065686 | 2.09E-07 | 1.02E-06 | Up | CUGCGACAAGUAAUUCCGAACGGA | 24 |
| tae-miR9776 | 2,996 | 3,147 | 0.186893606 | 3.30E-07 | 1.52E-06 | Up | UUGGACGAGGAUGUGCAACUG | 21 |
| tae-miR9668-5p | 554 | 679 | 0.40947965 | 6.43E-07 | 2.81E-06 | Up | CCAAUGACAAGUAUUUUCGGA | 21 |
| tae-miR167a | 62,972 | 56,678 | −0.035967586 | 1.22E-06 | 5.08E-06 | Down | UGAAGCUGCCAGCAUGAUCUA | 21 |
| tae-miR156 | 195 | 101 | −0.833164779 | 1.29E-06 | 5.11E-06 | Down | UGACAGAAGAGAGUGAGCACA | 21 |
| tae-miR167c-5p | 63,072 | 56,855 | −0.0337584 | 5.10E-06 | 1.93E-05 | Down | UGAAGCUGCCAGCAUGAUCUGC | 22 |
| List of differential expressed wheat miRNAs between FS3 and SS3 | ||||||||
| miR_name | FS3-reads | SS3-reads | log2Ratio(SS3/FS3) normalized | p-value | q-value (Storey and Tibshirani, 2003) | UP_Down | Sequences | Length |
| tae-miR156 | 23,134 | 179 | −8.522028941 | 0 | 0 | Down | UGACAGAAGAGAGUGAGCACA | 21 |
| tae-miR9672b | 20 | 50,329 | 9.789056616 | 0 | 0 | Up | UACCACGACUGUCAUUAAGCA | 21 |
| tae-miR9677a | 12 | 297,26 | 9.766357621 | 0 | 0 | Up | UGGCCGUUGGUAGAGUAGGAGA | 22 |
| tae-miR9666a-3p | 10 | 26,563 | 9.867084776 | 0 | 0 | Up | CGGUAGGGCUGUAUGAUGGCGA | 22 |
| tae-miR167a | 34,188 | 56,070 | −0.794378559 | 0 | 0 | Down | UGAAGCUGCCAGCAUGAUCUA | 21 |
| tae-miR167c-5p | 34,240 | 56,229 | −0.792485913 | 0 | 0 | Down | UGAAGCUGCCAGCAUGAUCUGC | 22 |
| tae-miR5062-5p | 7 | 19,573 | 9.941104754 | 0 | 0 | Up | UGAACCUUAGGGAACAGCCGCAU | 23 |
| tae-miR164 | 3,864 | 779 | −3.818517462 | 0 | 0 | Down | UGGAGAAGCAGGGCACGUGCA | 21 |
| tae-miR5048-5p | 4 | 4,666 | 8.679852991 | 0 | 0 | Up | UUUGCAGGUUUUAGGUCUAAGU | 22 |
| tae-miR9776 | 4 | 3,667 | 8.332266949 | 0 | 0 | Up | UUGGACGAGGAUGUGCAACUG | 21 |
| tae-miR9674b-5p | 4 | 3,415 | 8.229552262 | 0 | 0 | Up | AUAGCAUCAUCCAUCCUACCC | 21 |
| tae-miR9666b-3p | 0 | 5,158 | 11.82447846 | 0 | 0 | Up | CGGUUGGGCUGUAUGAUGGCGA | 22 |
| tae-miR9662a-3p | 1 | 2,934 | 10.01053555 | 0 | 0 | Up | UUGAACAUCCCAGAGCCACCG | 21 |
| tae-miR9662b-3p | 1 | 2,934 | 10.01053555 | 0 | 0 | Up | UGAACAUCCCAGAGCCACCGG | 21 |
| tae-miR9663-5p | 0 | 1,938 | 10.41223525 | 5.44E-203 | 2.68E-202 | Up | AAGCGUAGUCGAACGAAUCUG | 21 |
| tae-miR9672a-3p | 0 | 1,395 | 9.937931805 | 2.37E-157 | 1.09E-156 | Up | CCACGACUGUCAUUAAGCAUC | 21 |
| tae-miR9772 | 1 | 1,182 | 8.698896719 | 5.88E-157 | 2.56E-156 | Up | UGAGAUGAGAUUACCCCAUAC | 21 |
| tae-miR1130b-3p | 1 | 721 | 7.985737848 | 5.52E-104 | 2.27E-103 | Up | UCUUAUAUUAUGGGACGGAGG | 21 |
| tae-miR7757-5p | 1 | 656 | 7.849434403 | 5.57E-96 | 2.17E-95 | Up | AUAAAACCUUCAGCUAUCCAUC | 22 |
| tae-miR9670-3p | 0 | 737 | 9.017403208 | 5.92E-95 | 2.19E-94 | Up | AGGUGGAAUACUUGAAGAAGA | 21 |
| tae-miR9668-5p | 0 | 653 | 8.84282158 | 3.92E-86 | 1.38E-85 | Up | CCAAUGACAAGUAUUUUCGGA | 21 |
| tae-miR6201 | 2 | 535 | 6.55527748 | 1.05E-85 | 3.53E-85 | Up | UGACCCUGAGGCACUCAUACCG | 22 |
| tae-miR159b | 256 | 104 | −2.807677883 | 1.19E-78 | 3.76E-78 | Down | UUUGGAUUGAAGGGAGCUCUG | 21 |
| tae-miR159a | 256 | 104 | −2.807677883 | 1.19E-78 | 3.76E-78 | Down | UUUGGAUUGAAGGGAGCUCUG | 21 |
| tae-miR1135 | 0 | 457 | 8.327932754 | 2.17E-64 | 6.41E-64 | Up | CUGCGACAAGUAAUUCCGAACGGA | 24 |
| tae-miR9677b | 0 | 299 | 7.715884073 | 2.94E-45 | 8.37E-45 | Up | CAGGGCGGGGAACAGGUGGCC | 21 |
| tae-miR9676-5p | 0 | 261 | 7.519788395 | 2.33E-40 | 6.39E-40 | Up | UGGAUGUCAUCGUGGCCGUACA | 22 |
| tae-miR5084 | 0 | 186 | 7.03104121 | 3.13E-30 | 8.27E-30 | Up | AUACAGUACUGCAGAGGAUCCUAA | 24 |
| tae-miR397-5p | 0 | 178 | 6.96761583 | 4.15E-29 | 1.06E-28 | Up | UCACCGGCGCUGCACACAAUG | 21 |
| tae-miR1122c-3p | 0 | 150 | 6.720701089 | 4.22E-25 | 1.04E-24 | Up | UCUAAUAUUAUGGGACGGAGG | 21 |
| tae-miR9658-3p | 0 | 137 | 6.589914482 | 3.40E-23 | 8.11E-23 | Up | AUCGUUCUGGGUGAAUAGGCC | 21 |
| tae-miR9679-5p | 1 | 133 | 5.547164834 | 6.37E-23 | 1.47E-22 | Up | CAGAACCAGAAUGAGUAGCUC | 21 |
| tae-miR1137b-5p | 0 | 133 | 6.547164834 | 1.33E-22 | 2.98E-22 | Up | UCCGUUCCAGAAUAGAUGACC | 21 |
| tae-miR5175-5p | 0 | 113 | 6.312061361 | 1.36E-19 | 2.96E-19 | Up | UUCCAAAUUACUCGUCGUGGU | 21 |
| tae-miR1127b-3p | 0 | 104 | 6.192322117 | 3.29E-18 | 6.95E-18 | Up | ACAAGUAUUUCUGGACGGAGG | 21 |
| tae-miR160 | 159 | 193 | −1.228543519 | 1.70E-17 | 3.49E-17 | Down | UGCCUGGCUCCCUGUAUGCCA | 21 |
| tae-miR1120c-5p | 0 | 98 | 6.106592243 | 2.81E-17 | 5.62E-17 | Up | UAAUAUAAGAACGUUUUUGAC | 21 |
| tae-miR1120b-3p | 0 | 97 | 6.091795241 | 4.03E-17 | 7.85E-17 | Up | UUCUUAUAUUGUGGGACAGAG | 21 |
| tae-miR9674a-5p | 0 | 87 | 5.934825895 | 1.52E-15 | 2.88E-15 | Up | GCAUCAUCCAUCCUACCAUUC | 21 |
| tae-miR9669-5p | 0 | 79 | 5.795663147 | 2.90E-14 | 5.36E-14 | Up | UACUGUGGGCACUUAUUUGAC | 21 |
| tae-miR1120a | 0 | 72 | 5.6618074 | 3.96E-13 | 7.15E-13 | Up | ACAUUCUUAUAUUAUGAGACGGAG | 24 |
| tae-miR9653a-3p | 0 | 68 | 5.57934524 | 1.79E-12 | 3.16E-12 | Up | UUUGAGACUUUGGCCAUGGCC | 21 |
| tae-miR319 | 21 | 0 | −6.900435024 | 8.05E-11 | 1.39E-10 | Down | UUGGACUGAAGGGAGCUCCCU | 21 |
| tae-miR9775 | 0 | 55 | 5.273242112 | 2.64E-10 | 4.39E-10 | Up | UGUGCGCAAUAAGAUUUUGCUA | 22 |
| tae-miR1125 | 0 | 55 | 5.273242112 | 2.64E-10 | 4.39E-10 | Up | AACCAACGAGACCAACUGCGGCGG | 24 |
| tae-miR9657a-3p | 0 | 52 | 5.192322117 | 8.51E-10 | 1.37E-09 | Up | UGUGCUUCCUCGUCGAACGGU | 21 |
| tae-miR1117 | 0 | 47 | 5.04647125 | 6.10E-09 | 9.50E-09 | Up | UAGUACCGGUUCGUGGCACGAACC | 24 |
| tae-miR1136 | 0 | 47 | 5.04647125 | 6.10E-09 | 9.50E-09 | Up | UUGUCGCAGGUAUGGAUGUAUCUA | 24 |
| tae-miR398 | 19 | 5 | −3.43411702 | 2.02E-08 | 3.05E-08 | Down | UGUGUUCUCAGGUCGCCCCCG | 21 |
| tae-miR9782 | 0 | 38 | 4.739809912 | 2.24E-07 | 3.32E-07 | Up | GUAUUAGGUUGGUCAAAUUGACGA | 24 |
| tae-miR6197-5p | 0 | 37 | 4.701335764 | 3.36E-07 | 4.88E-07 | Up | UCUGUAAACAAAUGUAGGACG | 21 |
| tae-miR1122b-3p | 0 | 36 | 4.6618074 | 5.05E-07 | 7.18E-07 | Up | AGACUUAUAUGUAGGAACGGA | 21 |
| tae-miR396-5p | 0 | 34 | 4.57934524 | 1.14E-06 | 1.59E-06 | Up | AACUGUGAACUCGCGGGGAUG | 21 |
| tae-miR5049-3p | 0 | 31 | 4.446078709 | 3.91E-06 | 5.31E-06 | Up | AAUAUGGAUCGGAGGGAGUAC | 21 |
| tae-miR9656-3p | 0 | 31 | 4.446078709 | 3.91E-06 | 5.31E-06 | Up | CUUCGAGACUCUGAACAGCGG | 21 |
| tae-miR9654b-3p | 0 | 26 | 4.192322117 | 3.11E-05 | 4.11E-05 | Up | UUCCGAAAGGCUUGAAGCGAAU | 22 |
| tae-miR2275-3p | 0 | 23 | 4.015444355 | 0.00010918 | 0.000141743 | Up | UUUGGUUUCCUCCAAUAUCUCG | 22 |
| tae-miR9661-5p | 0 | 20 | 3.813810494 | 0.000387067 | 0.000493844 | Up | UGAAGUAGAGCAGGGACCUCA | 21 |