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. 2017 Aug 9;7:7683. doi: 10.1038/s41598-017-08067-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the LAMP- and hinge-amplification reaction for the detection of PSTVd. Real-time amplification curves of the LAMP assay (black) and the Hinge-assay (red) at 65 °C for 100 minutes. Fluorescence values are normalized (a). Capillary electrophoresis of LAMP (black) and Hinge samples (red) is shown as electropherogram with fluorescence values vs. runtime. Peaks 1 and 2 represent the internal size-standard (35 bp and 5000 bp). The virtual gel image on the right depicts the amplified DNA products of both samples (b). The size marker ranges from 35 bp to 5000 bp. Both reactions contained 0.2 ng of total input PSTVd DNA.