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. 2017 Aug 9;7:7683. doi: 10.1038/s41598-017-08067-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the time-to-positivity values of the amplification reactions with decreasing distance of the hinge-primer to the outer primer. (a) Binding sites of the primers are illustrated for the Hinge-forward (LZ-HF) and Hinge-reverse (LZ-HR) primer with their respective outer primer LZL-389/LZR-653, LZF/R-2 and LZF/R-0. (b) Time-to-positivity values demonstrate the relation of primer distances (length of amplified product) and amplification kinetics. Both orientations of forward hinge-primer (white bars) and the reverse hinge-primer (black bars) are shown. The experiments were carried out in triplicate and standard error bars are shown in the figure.